ammonium chloride Search Results


nh 4 cl  (MedChemExpress)
94
MedChemExpress nh 4 cl
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Nh 4 Cl, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/Ammonium+chloride%2C+for+cell+culture/bio_rxiv__2024__11__19__624259-60-13-21
Average 94 stars, based on 1 article reviews
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dimethyl  (Thermo Fisher)
94
Thermo Fisher dimethyl
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Dimethyl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/Dimethyloctadecyl%5B3-(trimethoxysilyl)propyl%5Dammonium+chloride%2C+60%25+in+methanol/arxiv__2603__12079-173-13-16
Average 94 stars, based on 1 article reviews
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ammonium chloride  (Loba Chemie)
86
Loba Chemie ammonium chloride
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Ammonium Chloride, supplied by Loba Chemie, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/ammonium+chloride/10__1039_slash_d5su00787a-54-13-17
Average 86 stars, based on 1 article reviews
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ammonium chloride solution aqueous  (Merck & Co)
86
Merck & Co ammonium chloride solution aqueous
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Ammonium Chloride Solution Aqueous, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/ammonium+chloride/pm41237771-130-168-185
Average 86 stars, based on 1 article reviews
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ammonium chloride  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ammonium chloride
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Ammonium Chloride, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/Ammonium+Chloride/bio_rxiv__413070-196-20-33
Average 93 stars, based on 1 article reviews
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benzalkonium chloride  (Valiant Co Ltd)
96
Valiant Co Ltd benzalkonium chloride
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Benzalkonium Chloride, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/Benzalkonium+chloride/bio_rxiv__64898__2026__04__04__716461-207-27-32
Average 96 stars, based on 1 article reviews
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ammonium chloride  (Aladdin Scientific Corporation)
94
Aladdin Scientific Corporation ammonium chloride
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Ammonium Chloride, supplied by Aladdin Scientific Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/Ammonium+chloride/pm36668904__es2c06327_si_001-75-17-37
Average 94 stars, based on 1 article reviews
ammonium chloride - by Bioz Stars, 2026-10
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bis  (Thermo Fisher)
86
Thermo Fisher bis
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Bis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/Bis(triphenylphosphoranylidene)ammonium+chloride%2C+97%25+(dry+wt%2E)%2C+water+%3C3%25/10__1039_slash_c9sc03386f-156-46-76
Average 86 stars, based on 1 article reviews
bis - by Bioz Stars, 2026-10
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n4 6 helium  (BOC Sciences)
90
BOC Sciences n4 6 helium
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
N4 6 Helium, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/Dodecyl+trimethyl+ammonium+chloride/10__3390_slash_plasma1010008-102-7-10
Average 90 stars, based on 1 article reviews
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acetylcholine chloride ach  (Toronto Research Chemicals)
92
Toronto Research Chemicals acetylcholine chloride ach
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Acetylcholine Chloride Ach, supplied by Toronto Research Chemicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ammonium+chloride/Acetylcholine+Chloride/pm20168046-37-0-11
Average 92 stars, based on 1 article reviews
acetylcholine chloride ach - by Bioz Stars, 2026-10
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dodecyl trimethyl ammonium chloride  (Aladdin Scientific Corporation)
93
Aladdin Scientific Corporation dodecyl trimethyl ammonium chloride
(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or <t>NH</t> <t>4</t> Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.
Dodecyl Trimethyl Ammonium Chloride, supplied by Aladdin Scientific Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or NH 4 Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.

Journal: bioRxiv

Article Title: PTBP1 is upregulated by Zika virus infection via HIF-1α signal and hijacks NS1 protein to induce NS1 degradation to restrain viral replication

doi: 10.1101/2024.11.19.624259

Figure Lengend Snippet: (A) HEK293T cells were transfected with the HA-Vector (2.0, 1.75, 1.5, 1.25, and 0.75 μg) and FLAG-Vector (1.0, 0, 0, 0, 0 μg) or FLAG-NS1 (0, 0, 0, 0, 0.5 μg), or FLAG-NS5 (0, 0, 0, 0, 0.5 μg) or HA-PTBP1 (0, 0, 0.25, 0.5, 1.0) along with eGFP-C1 (0, 0.25, 0.25, 0.25, 0.25 μg) plasmids. After 30 h, the cells were collected and subjected to Western blot analysis. (B) The scheme of two main models of protein degradation: UPS (ubiquitin-proteasome system) and ALP (autophagy-lysosome pathway). (C and D) HEK293T cells (C or A549 cells (D) were transfected with 1.5 μg of each plasmid: HA-Vector, FLAG-NS1, and HA-PTBP1, along with FLAG-NS1. At 18 h post-transfection, the cells were treated with DMSO, 3-MA (1.0 mM), MG132 (2.5 μM), or NH 4 Cl (5 mM) for another 12 h. The protein levels were analyzed by Western blot. (E) A549 cells were transfected with 1.0 μg of each plasmid: HA-Vector or HA-PTBP1 along with FLAG-NS1. At 24 h post-transfection, the cells were labeled with anti-FLAG and anti-HA primary antibodies followed by corresponding fluorescent secondary antibodies. The lysosomes were stained with Lyso-Tracker, while nucleus was stained with DAPI. The images displaying NS1 (red), PTBP1 (green), Lysosome (white), and DAPI (blue) were captured under a confocal fluorescence microscope. Scale bar = 20 μm.

Article Snippet: IOX2 (Cat: HY-15468), YC-1 (Cat: HY-14927), 3-MA (Cat: HY-19312), MG132 (Cat: HY-13259) and NH 4 Cl (Cat: HY-Y1269C) were purchased from MedChemExpress (Princeton, NJ, USA).

Techniques: Transfection, Plasmid Preparation, Western Blot, Ubiquitin Proteomics, Labeling, Staining, Fluorescence, Microscopy